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KMID : 0370220030470040234
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Yakhak Hoeji
2003 Volume.47 No. 4 p.234 ~ p.238
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Comparison of Linarin and Its Acetylated linarin for the Cytotoxicity on the Proliferation of Lymphocytes and Activation of Macrophages
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한신하/Han SH
신지혜/소명아/한혜란/김민준/임동술/김경제/Shin JH/So MA/Han HR/Kim MJ/Yim DS/Kim KJ
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Abstract
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Linarin is a main compound from Chrysanthemum zawadskii var, latilobum. However, the biological mechanisms of these activities are unclear, Because of this wide diversity of effects, it is believed that they may be exerted through pluripotent effectors of linarin. In our previous screening study, the effects of linarin on the mouse macrophages cell line, RAW 264.7 cells, were investigated. It was found that linarin could stimulate macrophages activation by the production of tumor necrosis factor (TNF). The linarin (6.25~12.5 μg/ml) inhibited the production of NO in LPS-activated RAW 264.7 cells and linarin became an useful candidates for the development of new drug to treat endotoxemia and inflammation accompanied by the overproduction of NO. However, linarin-treated total lymphocyte showed cytotoxicity in a dose dependent manner between 20 μg/ml and 40 μg/nl. In this study, linarin derivative (acetylated linarin) was synthesized in order to obtain less-cytotoxicity of linarin and evaluated for their in vitro cytotoxic activity against mouse total lymphocyte. There was no cytotoxic activity in a dose dependent manner (20-40 μ g/ml of acetylated linarin whereas linarin showed. The production of NO, however was not the case by this modified linarin. The cell morphological change was not significantly changed in response to acetylated linarin alone and these effects were potentiated by the addition of LPS. These results suggest that acetylated linarin may be developed to be a promising new drug candidate without cytotoxicity on the basis of its activity of macrophage activation.
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